I agree with Biffo that you would be unlikely to confuse "No." with the phrase "No" at the time you consider it. But I do occasionally practical experience a split-2nd hesitation when reading through a sentence with "No.", specifically if there is a space between it and the next variety: https://90sgirlgroupnoscrubs01223.techionblog.com/36559246/5-simple-techniques-for-90s-girl-group-no-scrubs