Retry the protocol with freshly produced dialysis buffer, becoming very careful so as to add the right amount of NaCl This is termed contact inhibition and it stops the density in the cells from becoming far too significant. To avoid contact inhibition, cells from the key cell tradition must be https://www.facebook.com/NewTechFind/posts/pfbid0TizBnHeTUt4yFdnb3DzF2WaHdHLwpDvZs16v9ymGH41REFXiJfUaWsbbWJg1qvcSl